Protocol 4: Gene Mapping of Recessive Mutant Phenotype
6:17
Protocol 5: DNA Sequencing and Analysis
7:08
Protocol 6: Further Analysis of Candidate Gene Function
7:46
Results: Climbing Pass Rate, Histological Screen, and Deficiency Mapping
9:11
Conclusion
副本
The main goal of this protocol is to use the power of forward genetics to identify genes which when dysregulated lead to neurodegeneration. The main advantage of this technique is that it provides an unbiased and straightforward approach for the i
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We present a protocol using a forward genetic approach to screen for mutants exhibiting neurodegeneration in Drosophila melanogaster. It incorporates a climbing assay, histology analysis, gene mapping and DNA sequencing to ultimately identify novel genes related to the process of neuroprotection.