Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
2:13
Time-Lapse Imaging
3:03
Image Analysis
5:00
Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
6:38
Conclusion
副本
Our protocol can be used to investigate the motility and localization of astrocytic organelles or proteins under controlled extracellular environments and mild disease states. Unlike the MHA fixed preparations, which provide single snapshots of pr
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Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.