Our protocol uses regular fluorophore probes and simple specimen preparation techniques that maintain the imaged cell in physiological condition to study highly dynamic cellular process and detailed sub-cellular structures. This technique allows u
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Presented here is a protocol for super-resolution live-cell imaging in intact tissue. We have standardized the conditions for imaging a highly sensitive adult stem cell population in its native tissue environment. This technique involves balancing temporal and spatial resolution to allow for the direct observation of biological phenomena in live tissue.