Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
2:51
Preparation of Cells for hMMTs Seeding
5:24
Seeding hMMTs
7:24
Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
8:57
Conclusion
副本
Our protocol describes detailed methods to fabricate and analyze a 3D human skeletal muscle microtissue culture. Muscle microtissues can be applied to studies of basic muscle biology, disease modeling, or for candidate molecule testing. The main a
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This manuscript describes a detailed protocol to produce arrays of 3D human skeletal muscle microtissues and minimally invasive downstream in situ assays of function, including contractile force and calcium handling analyses.