Preparation of Mitoplasts with a French Press for Mechanical Rupture of the OMM
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Electrophysiological Recordings of H+ Leak Through UCP1 and AAC
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Results: The Analysis of FA-Dependent Proton Current via UCP1 in Brown Fat and via AAC in Heart Mitoplast
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Conclusion
副本
This protocol describes how the patch clamp technique can be used to study the thermogenic capacity of mitochondria by directly measuring mitochondrial proton leak across the inner mitochondrial membrane. The direct measurement of the proton curre
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This method article details the main steps in measuring H+ leak across the inner mitochondrial membrane with the patch-clamp technique, a new approach to study the thermogenic capacity of mitochondria.