Imaging of EWS-FLI1 Condensation Formation on DNA Curtains
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Results: EWS-FLI1 Condensate Formation on Lambda DNA and Binding Events of the Detached Domain of mCherry-EWS-FLI1 on 25 GGAA Domain Repeats
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Conclusion
副本
We provide a single molecule assay to observe the phase separation of transcription factors on DNA This technique allows realtime realization of the dynamic process of transcription factors assembling as a liquid droplet on DNA. EWS-FL1 is one of
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Here, we describe the use of the single-molecule imaging method, DNA Curtains, to study the biophysical mechanism of EWS-FLI1 condensates assembling on DNA.