Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
3:37
Annexin V-FITC/PI Assay for Apoptosis Rate
5:00
Immunofluorescence Detection of Caspase-3 Generation
6:16
Immunofluorescence Assay of ROS Level
6:50
Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
8:11
Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
10:01
Conclusion
副本
The protocol in this study provides a combination strategy of two herbs for treating hypoxia-damaged PC12 cells, and a reference for optimizing the best combination mode of herbs. This technique can provide a simple, reliable, and efficient in vit
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This protocol provides a combination strategy of two herbs to treat injured PC12 cells. The protocol provides a reference for optimizing the best application mode of traditional Chinese medicine (TCM).
