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JoVE Journal
Medicine
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Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
1.2K Views
•
10:33 min
•
November 18th, 2022
DOI :
November 18th, 2022
•Rozdziały w tym wideo
0:04
Introduction
0:56
Cell Viability Assay
3:02
Screening of Optimal Combination of Two Drugs and its Protective Effect Evaluation
3:37
Annexin V-FITC/PI Assay for Apoptosis Rate
5:00
Immunofluorescence Detection of Caspase-3 Generation
6:16
Immunofluorescence Assay of ROS Level
6:50
Western Blot Detection of Protein Expression of Nrf2, p-Akt, Akt, Bcl-2, and Bax
8:11
Results: Effects of Drug Combinations on the Normal and Injured PC12 Cells
10:01
Conclusion
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