Fluorescence‐Activated Cell Sorting (FACS) of the Protoplasts
4:23
Smart‐Seq2 Library Preparation
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Results: Protoplast Isolation and Transcriptome Analysis
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Conclusion
副本
Transcriptome analysis on single cell or cell types can detect the subtle difference of gene expression being masked by heterogeneity, which is a powerful tool to uncover the elaborate intracellular regulatory mechanisms. Fluorescence activated ce
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The feasibility and effectiveness of high-throughput scRNA-seq methods herald a single-cell era in plant research. Presented here is a robust and complete procedure for isolating specific Arabidopsis thaliana root cell types and subsequent transcriptome library construction and analysis.