突变全小鼠胚胎的单细胞 RNA 测序:从上胚层到原肠胚形成结束
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09:14 min
June 14th, 2024
DOI :10.3791/66866-v
June 14th, 2024
Elizabeth Abraham1, Mikel Zubillaga1, Thomas Roule2, Eleonora Stronati3, Naiara Akizu2, Conchi Estaras1
1Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, 2Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 3Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia
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此视频中的章节
0:00
Introduction
1:50
Isolation of Murine Embryos During Gastrulation for Single‐Cell RNA Sequencing
5:30
Same‐Day Genotyping of Isolated Gastrulating Murine Embryos
7:22
Cell Dissociation of Isolated Gastrulating Murine Embryos for Single‐Cell Sequencing
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