Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
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09:14 min
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June 14th, 2024
DOI :
June 14th, 2024
•Elizabeth Abraham1, Mikel Zubillaga1, Thomas Roule2, Eleonora Stronati3, Naiara Akizu2, Conchi Estaras1
1Department of Cardiovascular Sciences, Aging + Cardiovascular Discovery Center, Temple University, Lewis Katz School of Medicine, 2Raymond G. Perelman Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 3Department of Child and Adolescence Psychiatry, Children's Hospital of Philadelphia
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Chapters in this video
0:00
Introduction
1:50
Isolation of Murine Embryos During Gastrulation for Single‐Cell RNA Sequencing
5:30
Same‐Day Genotyping of Isolated Gastrulating Murine Embryos
7:22
Cell Dissociation of Isolated Gastrulating Murine Embryos for Single‐Cell Sequencing
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