Anmelden

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

53.1K Views

12:44 min

December 3rd, 2014

December 3rd, 2014

53,073 Views

0:05

Title

1:28

Preparation of Protein Extracts from Cultured Cells

3:05

Preparation of Protein Extracts from Mouse Liver and Spleen

4:56

Synthesis of Radiolabeled IRE-containing RNA Probe

5:55

Purification of Radiolabeled IRE-containing RNA Probe

7:48

Electrophoretic Mobility Shift Assay (EMSA)

10:27

Results: The Iron-dependence of IRE/IRP Interactions

12:17

Conclusion

Here we present a protocol to analyze RNA/protein interactions. The electrophoretic mobility shift assay (EMSA) is based on the differential migration of RNA/protein complexes and free RNA during native gel electrophoresis. By using a radiolabeled RNA probe, RNA/protein complexes can be visualized by autoradiography.

Wir verwenden Cookies, um Ihre Erfahrung auf unserer Website zu verbessern.

Indem Sie unsere Website weiterhin nutzen oder auf „Weiter“ klicken, stimmen Sie zu, unsere Cookies zu akzeptieren.