Agarose Embedding and Vibratome Sectioning of Thymic Lobes
5:30
Preparation of Thymocytes for Overlaying
6:59
Overlaying Thymocytes onto the Thymic Slices
7:48
Results: Positive and Negative Selection of Thymocytes
9:29
Conclusion
Transkript
The overall goal of this procedure is to prepare thymic slices that in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. This method can help answer key questions about T cell development,
Sign in or start your free trial to access this content
We describe the preparation of thymic slices that, in combination with flow cytometry, can be used to model positive and negative selection of developing T cells. Thymic slices can also be adapted for the in situ analysis of thymocyte migration, localization, and signaling via immunofluorescence and two-photon microscopy.