Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
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Results: Mycobacterial mRNA Extraction Analysis
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Conclusion
Transkript
Mutant library screening and transcriptomic studies of intracellular pathogens can help answer key questions about genomic and regulatory adaptations of pathogens of interest that are used to survive inside the host. The main advantages of these t
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Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.