Transfection, Chemical Fixation and Peroxidase Reaction
3:48
High Pressure Freezing
4:46
Freeze Substitution
6:23
Resin Infiltration and Embedding
8:11
Sectioning and TEM Imaging
9:17
Results: TEM Images
11:07
Conclusion
Transkript
This method combines the ability to stain for a membrane protein while also preserving membrane integrity and subcellular architecture. So it's really powerful for precisely localizing a membrane associated protein. The main advantage of this tech
Melden Sie sich an oder starten Sie Ihre kostenlose Testversion, um auf diese Inhalt zuzugreifen
This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.