Results: Acquiring Nanoindentation Data on Hydrogels and Cells
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Conclusion
Transkript
This protocol shows a step-by-step guide to measure the stiffness of hydrogels and cells using a commercially available nanoindenter and also presents an open-source software to reproducibly analyze acquired data. The protocol allows us to obtain
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The protocol presents a complete workflow for soft material nanoindentation experiments, including hydrogels and cells. First, the experimental steps to acquire force spectroscopy data are detailed; then, the analysis of such data is detailed through a newly developed open-source Python software, which is free to download from GitHub.