Encapsulation of Bacteria with the Photosensitizer
2:07
In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
2:50
In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
3:51
Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
4:47
Conclusion
Transkript
The protocol presents how the combination of liquid cell TEM and light illumination can be used to observe the structural changes in the bacteria encapsulated with photosensitizer upon light illumination. The main advantage of this technique is it
Sign in or start your free trial to access this content
The present protocol describes transmission electron microscope (TEM) modifications with a light illumination system, the fabrication of liquid cells, and in situ TEM observations of light-induced interactions between bacterial cells and a photosensitizer. The sample preparation methods, electron beam damage, and imaging are also discussed.