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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

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12:21 min

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February 16th, 2009

DOI :

February 16th, 2009

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14,000 Views

Georgeann S. O'Brien¹, Sandra Rieger¹, Seanna M. Martin¹, Ann M. Cavanaugh¹, Carlos Portera-Cailliau², Alvaro Sagasti¹

¹Department of Molecular Cell and Developmental Biology, University of California, Los Angeles, ²Departments of Neurology and Neurobiology, University of California, Los Angeles

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Two photon Axotomy

Time lapse Confocal Imaging

Zebrafish Embryos

Cellular Mechanisms Of Development

Long term Imaging

Automated Image Capture

Confocal Microscope

Peripheral Sensory Axons

Femtosecond Laser

Two photon Microscope

Sensory Neuron Regeneration

Anesthetized Embryos

Agarose Positioning

Imaging Chamber

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Two-photon axotomy and time-lapse confocal imaging in live zebrafish embryos

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