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Liberase-Based Enzymatic Purification of Microglial Cells Isolated from a Mouse Spinal Cord

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02:42 min

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September 22nd, 2023

DOI :

10.3791/200616-v

September 22nd, 2023

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158 Views

Claudia I Gutiérrez-Román¹^,², María E Meléndez Camargo², Cuauhtémoc C García Rojas³, Miguel Jimenez Olvera⁴, Sandra H Gutiérrez Román⁵, Oscar Medina-Contreras¹

¹Epidemiology, Endocrinology & Nutrition, Mexico Children's Hospital, ²Pharmacy Department, National School of Biological Sciences, National Polytechnic Institute, ³Graduate Department, Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care, Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service, Metropolitan Angeles Hospital

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Keywords Microglia Isolation

Enzymatic Digestion

Density Gradient

Cell Purification

De la serie

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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