Day 1: Preparing German Glass Coverslips and Seeding Cells
3:16
Day 2: Fixing, Permeablilization, and Adding Primary Ab to Cells
12:47
Day 3: Washing, secondary Ab incubation, nuclear stain, and mountin
18:53
Conclusion
Immunocytochemistry is a powerful method to determine the presence, subcellular localization, and relative abundance of an antigen of interest in cultured cells. This protocol presents an easy-to-follow series of steps that will enable one to conserve antibodies and get the most out of one's staining.