We would like to thank members of the Tessier lab for critical reading of this manuscript and helpful suggestions during the preparation of this protocol.

1. El hambre <ol><li> Preparar volar viales de hambre por saturación de una bola de algodón con el 18,2 mO agua en el fondo de un vial mosca estándar. Por otra parte, de manera similar saturar una pequeña tira de papel de filtro con 18,2 mO agua y el lugar en un ángulo dentro del vial. </li><li> Recoger las moscas en conjuntos de ~ 100 animales en una almohadilla de CO 2 y luego agregar las moscas a un vial preparado. Nota: Los mejores resultados se obtienen con los animales que están a menos de 5 días de edad. Sin embargo, la edad exacta de los animales puede ser controlado como una variable experimental para determinar los cambios en la preferencia de sabor con el tiempo. </li><li> Utilice un tapón de algodón o espuma para asegurar los viales cerrados. Colocar los viales en su lado en una incubadora con ambiente controlado. Mantener la temperatura a 25 ° C, y la humedad por encima de 70%. Deja viales sin tocar durante 24 horas. </li></ol> Ensayo Preferencia 2. Sabor <ol><li> Preparar todos los estimulantes del gusto para el ensayo en el mismo día unas prueba. Nota: Los estimulantes del gusto exacta a usar variará dependiendo de la pregunta experimental que se les pide. Los siguientes son ejemplos de estimulantes del gusto utilizados en este protocolo. Ver la sección 4 para optimizaciones. <ol><li> Preparar el control saborizante (sacarosa 1 mM) mediante la combinación de 10 l de solución de sacarosa 100 mM, 13 l de colorante rojo, y 977 l de agua 18.2 mO. </li><li> Preparar saborizante experimental (sacarosa al 5 mM) mediante la combinación de 50 l de solución de sacarosa 100 mM, 10 l de colorante azul, y 940 l de agua 18.2 mO. </li></ol></li><li> Hacer cámaras de ensayo usando una 100 mm x 15 mm placa de petri de plástico estándar preparado de la siguiente manera: <ol><li> Coloque tres gotas 10 l de saborizante de control más cercano al borde de la placa a las 12 horas y las otras 3 gotas a las 6 h. Asegúrese de que la separación entre gotas es similar. </li><li> Coloque tres gotas 10 l de saborizante experimental más cercana al borde de la placa a las 3 en punto y unaotras 3 gotas a las 9 en punto. Asegúrese de que la separación entre gotas es similar. </li><li> Repita los pasos 2.2.1 y 2.2.2 para tantas repeticiones como se desee. </li></ol></li><li> 1 frasco de vacío ~ 100 moscas hambrientas en una almohadilla de CO 2 sólo el tiempo suficiente para anestesiar a todos los animales (aproximadamente 10 segundos). Cepillo de los animales en el medio de una cámara de ensayo preparado y cubrir con la tapa de la placa. Nota: El aumento de los periodos de exposición al CO 2 se debe evitar para mejorar el tiempo de recuperación y limitar la interferencia con el comportamiento alimentario. La exposición a hielo (~ 5 min) puede ser utilizado para anestesiar para evitar CO 2 efectos de comportamiento que pueden surgir de incluso una exposición limitada. </li><li> Coloque la cámara de ensayo en una caja de cartón opaco. Asegúrese de marcar la parte exterior de la caja con la condición y el genotipo siendo probados. </li><li> Coloque la configuración completa (cámara de ensayo contenida dentro de la caja de cartón desde el paso 2.4) en un 25 ° C incubadora con al menos un 70% de humedad durante 2 horas. </li&gt; <li> Repita los pasos 2.3 a través 2.5 para todas las repeticiones. </li><li> Después de 2 horas, colocar las cámaras de ensayo, todavía contenidas dentro de cajas de cartón, directamente en un congelador a -20ºC hasta que esté listo para la cuantificación. </li></ol> 3. Ensayo de Sabor Preferencia Cuantificación <ol><li> Permitir que una sola cámara de ensayo se caliente a la temperatura ambiente (aproximadamente 5 minutos). </li><li> Bajo un microscopio de disección, usando un cepillo o un par de pinzas, los animales del grupo basado en el color de su abdomen: rojo, azul, púrpura o clara (Figura 1). </li><li> Registre el número de animales en cada grupo. Considere animales claras para no hayan participado en el ensayo y por lo tanto no los incluyen en los cálculos. </li><li> Calcular el índice de preferencia de acuerdo con una de las siguientes ecuaciones: <ol><li> Si se añade el saborizante experimental de interés para el tinte rojo, a continuación, utilizar (N + rojo púrpura 0,5 N) / (N + N rojo azul + N purple). </li><li> Si se añade el saborizante experimental para el colorante azul, y luego ajustar la ecuación de (N + azul púrpura 0,5 N) / (N + N azul rojo púrpura + N). </li></ol></li><li> Repetir los cálculos para todas las condiciones experimentales y repeticiones. </li></ol> 4. Optimización de Ensayo de preferencia de sabor <ol><li> Empíricamente determinar la concentración de los indicadores de colorante de alimentos para ser usado de modo colorante de alimentos no afecta el resultado del ensayo de sabor, como sigue: <ol><li> Preparar 4 estimulantes del gusto usando el mismo compuesto de base (por ejemplo, sacarosa al 5 mM) como se indica en el paso 2.1, pero omitir el colorante de alimentos. </li><li> Añadir 1,3% de colorante rojo a uno de los estimulantes del gusto. Hacer los 3 restantes con estimulantes del gusto azul colorante de alimentos de las concentraciones que varían en cada tubo (por ejemplo, 0,6%, 1% y 1,3%). </li><li> protocolo completo los pasos 2.2 a 3.4 para cada par saborizante: 1,3% frente a 0,6% de azul rojo; 1,3% frente a 1% de color rojo y azul1,3% frente a 1,3% de azul rojo. </li><li> Repita el paso 4.1.1-4.1.3 con diferentes porcentajes de colorante azul hasta los promedios del índice de preferencia un valor de 0 (Figura 2). Nota: Como punto de partida, un 1,3% de colorante rojo junto con 1% de colorante azul normalmente da buenos resultados. Si hay concentración satisfactoria de colorante azul se puede adaptar al 1,3% de colorante, a continuación, paso 4.1.1 a través de 4.1.3 se puede repetir con diferentes concentraciones de colorante rojo y una concentración constante de colorante azul. </li><li> Analizar todas las condiciones de la prueba con las mismas concentraciones de colorantes de alimentos optimizados. </li></ol></li></ol>

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The authors declare that they have no competing financial interests.

Hemos descrito un protocolo simple pero eficaz para determinar preferencia de sabor en Drosophila. Las versiones de este ensayo se utilizan habitualmente en experimentos para determinar las contribuciones de los receptores gustativos (gr) para percibir las diferentes calidades (amargo, dulce, agrio, salado y umami) de los compuestos del sabor. El genoma de Drosophila contiene aproximadamente 60 genes que codifican los receptores gustativos 68 identificados por splicing alternativo 8,9. Sin e...

Los animales usan para distinguir chemosensation condiciones ventajosas, aparte de las condiciones desventajosas. Esta percepción puede ser crítico para cosas tales como la determinación de la mejor fuente de alimento, evitar sustancias tóxicas o determinar el mejor socio de acoplamiento 1. Chemosensation a menudo se divide en dos componentes sensoriales: sentidos olfativos y gustativos sentidos. Un principal característica distintiva de estos sentidos es que el olfato (olor) se utiliza para muestrear el ambiente químico gaseoso circundante mientras gustation (sabor) requiere contacto físico con un sustrato no volátil. Ambas modalidades sensoriales estimulan respuestas neurológicas que son tratados y decodificados en el cerebro para producir el comportamiento de atracción o repulsión adecuada 2. Estos sentidos son de importancia crítica para la supervivencia del animal. La mosca de la fruta Drosophila melanogaster es un organismo modelo que sigue creciendo en popularidad para su uso en entendering cómo los insectos perciben olor y sabor. moscas de la fruta ofrecen enormes ventajas sobre otros sistemas modelo debido a la gran cantidad de herramientas genéticas disponibles para la disección de las vías moleculares, celulares y de comportamiento. Trabajo en los últimos 15 años ha sido especialmente importante en la caracterización de las identidades celulares específicos, receptores neuronales, y los mecanismos que intervienen en tanto olor y el sabor de señalización. Ahora, el poder de la genética de Drosophila se utiliza para aclarar aún más cómo estos procesos se codifican en el nivel sola neurona y simple circuito 3-6. Por lo tanto, los ensayos que proporcionan anotó fácilmente lecturas de las alteraciones en las vías sensoriales son vitales para el avance continuado de esos campos. Mientras que una gran cantidad se sabe acerca de cómo las señales olfativas son codificados y procesados ​​en el cerebro, y mucho menos se sabe acerca de los mecanismos similares en la vía gustativa. Se describe aquí un protocolo que se puede utilizar para determinar preferen gustativasce en Drosophila. Drosophila, como los mamíferos, por lo general prefieren compuestos de sabor dulce en lugar de compuestos de sabor amargo. Cualquier combinación de estas fuentes de alimentos se puede utilizar en este diseño experimental para determinar cómo las alteraciones genéticas conocidas afectan a la elección gusto. Además, las estrategias de intervención farmacológica de manera similar se pueden evaluar por sus efectos sobre la preferencia del gusto de los animales. La facilidad y flexibilidad de este ensayo hace que sea un paradigma útil para comprender la naturaleza de la percepción gustativa en Drosophila.

<table><tbody><tr><td>Blue Food Coloring (Water, Propylene Glycol, FD&amp;amp;C Blue 1 and Red 40, Propylparaben)</td><td>McCormick</td><td>N/A</td><td></td></tr><tr><td>Cryo/Freezer Boxes w/o Dividers</td><td>Fisher</td><td>03-395-455</td><td></td></tr><tr><td>Dumont #5 Forceps</td><td>Fine Science Tools</td><td>11251-20</td><td></td></tr><tr><td>Glacial Acetic Acid</td><td>Fisher</td><td>BP2401-500</td><td></td></tr><tr><td>Leica S6 E Stereozoom 0.63X-4.0X microscope</td><td>W. Nuhsbaum, Inc.</td><td>10446294</td><td></td></tr><tr><td>Petri dish (100 mm x 15 mm)</td><td>BD Falcon</td><td>351029</td><td>Reuseable if thoroughly washed and dried</td></tr><tr><td>Quick-Snap Microtubes</td><td>Alkali Scientific Inc.</td><td>C3017</td><td></td></tr><tr><td>Red Food Coloring (Water, Propylene Glycol, FD&amp;amp;C Reds 40 and 3, Propylparaben)</td><td>McCormick</td><td>N/A</td><td></td></tr><tr><td>Sucrose</td><td>IBI Scientific</td><td>IB37160</td><td></td></tr></tbody></table>

taste preference assay for adult drosophila

Olfactory and gustatory perception of the environment is vital for animal survival. The most obvious application of these chemosenses is to be able to distinguish good food sources from potentially dangerous food sources. Gustation requires physical contact with a chemical compound which is able to signal through taste receptors that are expressed on the surface of neurons. In insects, these gustatory neurons can be located across the animal's body allowing taste to play an important role in many different behaviors. Insects typically prefer compounds containing sugars, while compounds that are considered bitter tasting are avoided. Given the basic biological importance of taste, there is intense interest in understanding the molecular mechanisms underlying this sensory modality. We describe an adult Drosophila taste assay which reflects the preference of the animals for a given tastant compound. This assay may be applied to animals of any genetic background to examine the taste preference for a desired soluble compound.

Algunos resultados típicos de los ensayos de preferencias gustativas se muestran a continuación. En la mayoría de los experimentos alguna variación en la intensidad de la coloración abdominal se verá (Figura 1). Cualquier coloración en el abdomen ya sea intensa o débil se considera una ingesta positivo. Por tanto, es aconsejable que los investigadores para anotar animales mientras ciegos a la condición experimental de modo que se limiten los posibles sesgos. </p...

Watch this Scientific Journal Video about Taste Preference Assay for Adult Drosophila at JoVE.com

Taste Preference Assay for Adult Drosophila

Taste is an important sensory process which facilitates attraction to beneficial substances and avoidance of toxic substances. This protocol describes a simple ingestion assay for determining Drosophila gustatory preference for a given chemical compound.

Ensayo Preferencia gusto por adulto<em&gt; Drosophila</em

Olfactory and gustatory perception of the environment is vital for animal survival.  The most obvious application of these chemosenses is to be able to ...

taste-preference-assay-for-adult-drosophila

Research

JoVE Journal

Neuroscience

Taste Preference Assay for Adult Drosophila

9.6K vistas.  Indiana University School of Medicine-South Bend. Taste is an important sensory process which facilitates attraction to beneficial substances and avoidance of toxic substances. This protocol describes a simple ingestion assay for determining Drosophila gustatory preference for a given chemical compound.

Video: Taste Preference Assay for Adult Drosophila

Appetitive Associative Olfactory Learning in Drosophila Larvae

In the following we describe the methodological details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with genetic interference, provides a handle to analyze the neuronal and molecular fundamentals of specifically associative learning in a simple larval brain.
Organisms can use past experience to adjust present behavior. Such acquisition of behavioral potential can be defined as learning, and the physical bases of these potentials as memory traces1-4. Neuroscientists try to understand how these processes are organized in terms of molecular and neuronal changes in the brain by using a variety of methods in model organisms ranging from insects to vertebrates5,6. For such endeavors it is helpful to use model systems that are simple and experimentally accessible. The Drosophila larva has turned out to satisfy these demands based on the availability of robust behavioral assays, the existence of a variety of transgenic techniques and the elementary organization of the nervous system comprising only about 10,000 neurons (albeit with some concessions: cognitive limitations, few behavioral options, and richness of experience questionable)7-10.
Drosophila larvae can form associations between odors and appetitive gustatory reinforcement like sugar11-14. In a standard assay, established in the lab of B. Gerber, animals receive a two-odor reciprocal training: A first group of larvae is exposed to an odor A together with a gustatory reinforcer (sugar reward) and is subsequently exposed to an odor B without reinforcement 9. Meanwhile a second group of larvae receives reciprocal training while experiencing odor A without reinforcement and subsequently being exposed to odor B with reinforcement (sugar reward). In the following both groups are tested for their preference between the two odors. Relatively higher preferences for the rewarded odor reflect associative learning - presented as a performance index (PI). The conclusion regarding the associative nature of the performance index is compelling, because apart from the contingency between odors and tastants, other parameters, such as odor and reward exposure, passage of time and handling do not differ between the two groups9.

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larvae are able to associate odor stimuli with gustatory reward. Here we describe a simple behavioral paradigm that allows the analysis of appetitive associative olfactory learning.

Apetitivo aprendizaje asociativo olfativa en<em&gt; Drosophila</em&gt; Las larvas

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

Investigación

Neurociencias

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

Un ensayo de una sola mosca por el comportamiento de forrajeo en<em&gt; Drosophila</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Testing Drosophila Olfaction with a Y-maze Assay

Detecting signals from the environment is essential for animals to ensure their survival. To this aim, they use environmental cues such as vision, mechanoreception, hearing, and chemoperception through taste, via direct contact or through olfaction, which represents the response to a volatile molecule acting at longer range. Volatile chemical molecules are very important signals for most animals in the detection of danger, a source of food, or to communicate between individuals. Drosophila melanogaster is one of the most common biological models for scientists to explore the cellular and molecular basis of olfaction. In order to highlight olfactory abilities of this small insect, we describe a modified choice protocol based on the Y-maze test classically used with mice. Data obtained with Y-mazes give valuable information to better understand how animals deal with their perpetually changing environment. We introduce a step-by-step protocol to study the impact of odorants on fly exploratory response using this Y-maze assay.

Testing Drosophila Olfaction with a Y-maze Assay

We present a choice test to reveal the influence of odorants on Drosophila behavior using a Y-maze assay.

Pruebas<em&gt; Drosophila</em&gt; El olfato con un ensayo del laberinto

Detecting signals from the environment is essential for animals to ensure their survival. To this aim, they use environmental cues such as vision, ...

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. When selecting sites to deposit their eggs, female flies are capable of ranking the relative attractiveness of their options and choosing the &#34;greater of two goods.&#34; However, most egg-laying preference assays are not practical if one wants to take a systematic genetic screening approach to search for the circuit basis underlying this simple decision-making process, as they are population-based and laborious to set up. To increase the throughput of studying of egg-laying preferences of single females, we developed custom chambers that each can simultaneously assay egg-laying preferences of up to thirty individual flies as well as a protocol that ensures each female has a high egg-laying rate (so that their preference is readily discernable and more convincing). Our approach is simple to execute and produces very consistent results. Additionally, these chambers can be equipped with different attachments to allow video recording the egg-laying animals and to deliver light for optogenetics studies. This article provides the blueprints for fabricating these chambers and the procedure for preparing the flies to be assayed in these chambers.

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

Ensayo de Alto Rendimiento para el Examen de Preferencias de la puesta de huevos individual<em&gt; Drosophila melanogaster</em

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools.

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

We describe a protocol for inducing rewarding and nonrewarding experiences in fruit flies (Drosophila melanogaster) using voluntary ethanol consumption as a measure for changes in reward states.

Una manera sencilla de medir alteraciones en el comportamiento de búsqueda de recompensa Uso<em&gt; Drosophila melanogaster</em

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We ...

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated.

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake.

El alimentador de ensayo capilar para analizar el consumo de alimentos en<em&gt; Drosophila melanogaster</em

For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

Un ensayo rápido de preferencia alimentaria en Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster

Source: Bovier, T. F., et al. <a href="https://www.jove.com/video/59535/" target="_blank">Methods to Test Endocrine Disruption in Drosophila melanogaster</a>. J. Vis. Exp. (2019).
This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).

Test de Alimentación: Evaluación de las Preferencias Alimentarias en Drosophila melanogaster

Source: Bovier, T. F., et al. Methods to Test Endocrine Disruption in Drosophila melanogaster. J. Vis. Exp. (2019).
This video describes a feeding assay ...

JoVE Encyclopedia of Experiments

Biología

Drosophila melanogaster (mosca de la fruta)

Adulto

Temperature Gradient Assay: A Method to Test Temperature Preference in Drosophila Larvae

Source: Liu, J., et al. <a href="https://www.jove.com/video/57963/" target="_blank">A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae</a>. J. Vis. Exp. (2018).
Drosophila fruit flies can distinguish small changes in temperature and therefore select environments with favorable thermal conditions. This video describes a behavioral assay that tests the temperature preference of Drosophila larvae on a linear thermal gradient.

Ensayo de gradiente de temperatura: un método para probar la preferencia de temperatura en larvas de Drosophila

Source: Liu, J., et al. A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae. J. Vis. Exp. (2018).
Drosophila fruit flies ...

Larva

Taste Preference Assay: A Method for Measuring Feeding Behavior in Drosophila

Source: Bantel, A. P. and Tessier, C. R. <a href="https://www.jove.com/video/54403/" target="_blank">Taste Preference Assay for Adult Drosophila</a>. J. Vis. Exp. (2016).
This video describes the taste preference assay, a behavioral method used to measure attraction or avoidance towards colored solutions that taste differently by assessing the fly&#39;s abdominal coloration after ingestion of the preferred substance. The featured protocol demonstrates the procedure used to measure flies&#39; preference towards solutions of varying sucrose concentrations.

Ensayo de preferencia gustativa: un método para medir el comportamiento alimentario en Drosophila

Source: Bantel, A. P. and Tessier, C. R. Taste Preference Assay for Adult Drosophila. J. Vis. Exp. (2016).
This video describes the taste preference ...

Ensayo Preferencia gusto por adulto

Resumen

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