We would like to thank members of the Tessier lab for critical reading of this manuscript and helpful suggestions during the preparation of this protocol.

1. 기아 <ol><li> 표준 플라이 유리 병의 하단에 18.2 MΩ 물 목화 공을 포화에 의해 기아 튜브를 비행 준비합니다. 다르게는, 유사 바이알 내의 각도 18.2 MΩ 물 장소 여과지의 작은 조각을 포화. </li><li> 준비된 유리 병에 파리를 추가 한 후 CO 2 패드 ~ 100 동물의 세트로 파리를 수집합니다. 참고 : 최상의 결과 미만 5 일 이전입니다 동물 얻을 수있다. 그러나, 동물의 실제 나이는 시간에 기본 맛의 변화를 결정하는 변수로 실험을 제어 할 수있다. </li><li> 유리 병 폐쇄 확보하기 위해 목화 공 또는 거품 스토퍼를 사용합니다. 환경 제어 배양기에서 그들의 편에서 튜브를 놓습니다. 25 ° C의 온도, 70 % 이상의 습도를 유지한다. 24 시간 동안 그대로 튜브를 남겨주세요. </li></ol> 2. 맛 선호도 분석 <ol><li> 같은 날 A의 분석에 대한 모든 tastants 준비의 테스트. 참고 : 실험 질문에 따라 달라집니다 사용되는 정확한 tastants이 요구된다. 다음은이 프로토콜에서 사용되는 예제 tastants 있습니다. 최적화를위한 부 (4)를 참조하십시오. <ol><li> 100 밀리미터 자당 용액 10 μL, 붉은 식용 색소의 13 μL, 18.2 MΩ 물 977 μl를 조합하여 제어 tastant (1 mM의 자당)을 준비합니다. </li><li> 100 밀리미터 자당 용액 50 μL, 파란색 식용 색소의 10 μL, 18.2 MΩ 물 940 μl를 결합하여 실험 tastant (5 MM의 자당)을 준비합니다. </li></ol></li><li> 다음과 같은 방법으로 제조 된 표준 100mm X 15mm의 플라스틱 페트리 접시를 사용하여 분석을 챔버 : <ol><li> 12시 방향 판의 가장자리에 가까운 제어 tastant 세 10 μl의 방울, 6시 방향에 다른 3 방울을 배치합니다. 방울 사이의 간격이 유사하다는 것을 확인하십시오. </li><li> 3시하고있는 플레이트의 가장자리에 가까운 실험 tastant 세 10 μL 방울을 배치다른 3 9시에 삭제합니다. 방울 사이의 간격이 유사하다는 것을 확인하십시오. </li><li> 원하는대로 반복 많은 복제에 대한 2.2.1와 2.2.2 단계를 반복합니다. </li></ol></li><li> 다만 충분히 모든 동물 (약 10 초)를 마취하는 CO 2 패드 상 ~ 100 굶주린 파리의 빈 1 병. 준비된 분석 챔버의 중앙으로 동물을 브러시와 접시의 뚜껑을 커버. 주 : CO 2 노광 장기간의 공급 동작과 복구 시간 제한 및 간섭을 개선하기 위해 피해야한다. 얼음에 노출 (~ 5 분)의 제한에도 노출로부터 발생하는 CO 2 행동 효과를 피하기 위해 마취를 위해 사용될 수있다. </li><li> 불투명 한 골판지 상자에 분석 챔버를 놓습니다. 조건과 유전자형이 테스트되고 함께 상자의 외부에 라벨을해야합니다. </li><li> 2 시간 동안 적어도 70 % 습도와 25 ° C 배양기에 전체 설치 (단계 2.4에서 골판지 상자에 포함 된 분석 챔버)를 놓습니다. </li&gt; <li> 반복 모든 복제 2.5을 통해 2.3 단계를 반복합니다. </li><li> 2시간 후, 직접 정량에 대한 준비가 될 때까지 -20 ° C 냉장고에 여전히 골 판지 상자에 포함 된 분석 챔버를 배치합니다. </li></ol> 3. 맛 특혜 분석의 정량화 <ol><li> 하나의 분석 챔버는 실온 (약 5 분)을 따뜻하게 할 수 있습니다. </li><li> 빨강, 파랑, 보라색 또는 클리어 (그림 1) : 해부 현미경, 브러시 또는 집게의 쌍을 사용하여, 그룹 동물 자신의 복부의 색상에 따라. </li><li> 각 그룹에서의 동물의 수를 기록한다. 어떤 계산에 포함하지 않는 때문에 분석에 참여하지 않은 명확한 동물을 고려합니다. </li><li> 하기 식 중 어느 한 항에있어서, 상기 선호 지수를 계산한다 : <ol><li> + N 블루 +의 N의 purpl 관심의 실험 tastant가 붉은 염료에 추가되면, 사용 (N 빨간색 + 0.5N 보라색) / (N 레드전자). </li><li> 실험 tastant가 파란색 염료에 추가되면, 다음 (N 블루 보라색 + 0.5N) / (N 블루 + N 빨간색 + N 보라색)에 식을 조정합니다. </li></ol></li><li> 모든 실험 조건 및 복제에 대한 계산을 반복합니다. </li></ol> 맛의 기본 설정 분석 4. 최적화 <ol><li> 경험적 식용 색소가 맛이 분석의 결과에 영향을주지 않도록 다음과 같이 사용되는 식용 색소 지표의 농도를 결정 : <ol><li> 단계 2.1로 표시된 동일한 염기 화합물 (예, 5 mM의 크로스)를 사용하여 4 tastants 준비했지만 식용 색소를 생략. </li><li> tastants 중 하나에 1.3 % 붉은 식용 색소를 추가합니다. 각 튜브에 다양한 농도의 파란색 식용 색소 (예 : 0.6 %, 1 %, 1.3 %)와 나머지 3 tastants합니다. </li><li> 전체 프로토콜은 각 tastant 쌍 3.4을 통해 2.2 단계 : 0.6 % 청색 1.3 %, 적색 대; 1.3 % 빨간색 대 1 % 파란색과1.3 % 빨간색 대 1.3 % 파란색. </li><li> 선호 지수 평균 때까지 파란색 식용 색소의 다른 비율을 0 (그림 2)의 값이 단계를 반복 4.1.1-4.1.3. 참고 : 시작 지점으로, 1 % 파란색 식용 색소와 결합 1.3 % 빨간색 식용 색소는 일반적으로 좋은 결과를 얻을 수 있습니다. 파란색 식용 색소의 만족스러운 농도가 1.3 % 염료와 일치 할 수없는 경우, 4.1.3을 통해 4.1.1이 붉은 색과 파란색 식용 색소의 일정한 농도의 다양한 농도로 반복 될 수있다 단계. </li><li> 같은 최적화 된 식용 색소의 농도 테스트 할 모든 조건을 분석합니다. </li></ol></li></ol>

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The authors declare that they have no competing financial interests.

우리는 초파리 취향 우선 순위를 결정하기위한 단순하지만 효율적인 프로토콜을 설명 하였다. 이 분석의 버전은 정기적으로 맛 화합물 (쓴맛, 단맛, 신맛, 짠맛,​​ 감칠맛과) 다른 특성을 지각에 미각 수용체 (GRS)의 기여를 결정하기 위해 실험에 사용된다. 초파리 게놈 대체 접합 8,9에 의해 68 식별 미각 수용체를 인코딩 약 60 유전자가 포함되어 있습니다. 그러나, 이러한 이...

동물 불리한 조건에서 떨어져 유리한 조건을 구별하는 chemosensation를 사용합니다. 이 인식은, 최고의 음식 소스를 결정하는 독성 물질을 피하거나 가장 짝짓기 파트너 일을 결정하는 등의 것들에 대한 중요 할 수 있습니다. 후각의 감각과 미각 감각 : Chemosensation은 종종 두 개의 감각 구성 요소로 나누어 져 있습니다. 이러한 감각의 주요 구별되는 특징은 후각 (냄새) 맛보기 (맛) 비 휘발성 기판과 물리적 접촉을 필요로하는 동안 주위의 가스 화학 환경을 샘플링하는 데 사용되는 것입니다. 두 감각 양식을 처리하고 적절한 매력적인 또는 척력이 동작을 생성하기 위해 뇌에서 디코딩되는 신경 반응을 자극한다. 이 감각은​​ 동물의 생존을위한 따라서 중요합니다. 초파리 비행 열매는 이해에 사용하기 위해 인기가 성장을 계속하고 모델 생물이다곤충은 냄새와 맛을 감지하는 방법을 보내고. 초파리 때문에, 분자 세포, 행동 경로의 해부에 사용할 유전 도구의 재산에 다른 모델 시스템에 비해 엄청난 장점을 제공한다. 지난 15 년 동안 작업은 특정 세포 정체성, 신경 수용체를 특성화하고, 냄새와 맛 모두에 관여하는 메커니즘을 신호에 특히 쓸모있다. 지금, 초파리 유전학의 힘은 더욱 이러한 프로세스는 하나의 신경 세포 및 단일 회로 레벨 3-6에서 코딩하는 방법을 명료하게 사용되고있다. 따라서, 제공하는 분석은 쉽게 감각 경로에 변화의 판독이 분야의 지속적인 사전에 매우 중요 얻었습니다. 많은 코딩 뇌에서 처리하는 방법 후각 신호에 대해 알려져 있지만, 훨씬 덜은 미각 경로와 유사한 메커니즘에 대한 이해된다. 우리는 여기서 맛 preferen을 확인하는 데 사용될 수있는 프로토콜을 설명쓴 맛 화합물 반대로 초파리. 초파리의 CE 마크는 포유 동물처럼, 일반적으로 달콤한 맛 화합물을 선호합니다. 이러한 음식 소스의 조합은 알려진 유전 적 변화가 맛 선택에 영향을 미치는 방법을 결정하기 위해이 실험 설계에 이용 될 수있다. 또한, 약리학 적 개입 전략은 유사 동물의 맛이 환경에 미치는 영향에 대해 평가 될 수있다. 이 분석의 용이성과 유연성 초파리 미각 지각의 특성을 이해하는 것이 유용에게 패러다임을 만든다.

<table><tbody><tr><td>Blue Food Coloring (Water, Propylene Glycol, FD&amp;amp;C Blue 1 and Red 40, Propylparaben)</td><td>McCormick</td><td>N/A</td><td></td></tr><tr><td>Cryo/Freezer Boxes w/o Dividers</td><td>Fisher</td><td>03-395-455</td><td></td></tr><tr><td>Dumont #5 Forceps</td><td>Fine Science Tools</td><td>11251-20</td><td></td></tr><tr><td>Glacial Acetic Acid</td><td>Fisher</td><td>BP2401-500</td><td></td></tr><tr><td>Leica S6 E Stereozoom 0.63X-4.0X microscope</td><td>W. Nuhsbaum, Inc.</td><td>10446294</td><td></td></tr><tr><td>Petri dish (100 mm x 15 mm)</td><td>BD Falcon</td><td>351029</td><td>Reuseable if thoroughly washed and dried</td></tr><tr><td>Quick-Snap Microtubes</td><td>Alkali Scientific Inc.</td><td>C3017</td><td></td></tr><tr><td>Red Food Coloring (Water, Propylene Glycol, FD&amp;amp;C Reds 40 and 3, Propylparaben)</td><td>McCormick</td><td>N/A</td><td></td></tr><tr><td>Sucrose</td><td>IBI Scientific</td><td>IB37160</td><td></td></tr></tbody></table>

taste preference assay for adult drosophila

Olfactory and gustatory perception of the environment is vital for animal survival. The most obvious application of these chemosenses is to be able to distinguish good food sources from potentially dangerous food sources. Gustation requires physical contact with a chemical compound which is able to signal through taste receptors that are expressed on the surface of neurons. In insects, these gustatory neurons can be located across the animal's body allowing taste to play an important role in many different behaviors. Insects typically prefer compounds containing sugars, while compounds that are considered bitter tasting are avoided. Given the basic biological importance of taste, there is intense interest in understanding the molecular mechanisms underlying this sensory modality. We describe an adult Drosophila taste assay which reflects the preference of the animals for a given tastant compound. This assay may be applied to animals of any genetic background to examine the taste preference for a desired soluble compound.

맛을 선호 분석에서 몇 가지 전형적인 결과는 다음과 같습니다. 대부분의 실험에서 복부 색상의 강도에 약간의 변화는 (그림 1) 볼 수 있습니다. 강한 또는 약한 여부 복부의 모든 색상이 양의 섭취로 간주됩니다. 잠재적 인 편견을 제한하기 위해 연구자들은 실험 조건에있는 동안 블라인드 동물을 득점하는 것은 그러므로 것이 좋습니다. <p class="jove_content" ...

Watch this Scientific Journal Video about Taste Preference Assay for Adult Drosophila at JoVE.com

Taste Preference Assay for Adult Drosophila

Taste is an important sensory process which facilitates attraction to beneficial substances and avoidance of toxic substances. This protocol describes a simple ingestion assay for determining Drosophila gustatory preference for a given chemical compound.

성인을위한 맛의 기본 설정 분석<em&gt; 초파리</em

Olfactory and gustatory perception of the environment is vital for animal survival.  The most obvious application of these chemosenses is to be able to ...

taste-preference-assay-for-adult-drosophila

Research

JoVE Journal

Neuroscience

Taste Preference Assay for Adult Drosophila

9.6K 조회수.  Indiana University School of Medicine-South Bend. Taste is an important sensory process which facilitates attraction to beneficial substances and avoidance of toxic substances. This protocol describes a simple ingestion assay for determining Drosophila gustatory preference for a given chemical compound.

비디오: Taste Preference Assay for Adult Drosophila

Appetitive Associative Olfactory Learning in Drosophila Larvae

In the following we describe the methodological details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with genetic interference, provides a handle to analyze the neuronal and molecular fundamentals of specifically associative learning in a simple larval brain.
Organisms can use past experience to adjust present behavior. Such acquisition of behavioral potential can be defined as learning, and the physical bases of these potentials as memory traces1-4. Neuroscientists try to understand how these processes are organized in terms of molecular and neuronal changes in the brain by using a variety of methods in model organisms ranging from insects to vertebrates5,6. For such endeavors it is helpful to use model systems that are simple and experimentally accessible. The Drosophila larva has turned out to satisfy these demands based on the availability of robust behavioral assays, the existence of a variety of transgenic techniques and the elementary organization of the nervous system comprising only about 10,000 neurons (albeit with some concessions: cognitive limitations, few behavioral options, and richness of experience questionable)7-10.
Drosophila larvae can form associations between odors and appetitive gustatory reinforcement like sugar11-14. In a standard assay, established in the lab of B. Gerber, animals receive a two-odor reciprocal training: A first group of larvae is exposed to an odor A together with a gustatory reinforcer (sugar reward) and is subsequently exposed to an odor B without reinforcement 9. Meanwhile a second group of larvae receives reciprocal training while experiencing odor A without reinforcement and subsequently being exposed to odor B with reinforcement (sugar reward). In the following both groups are tested for their preference between the two odors. Relatively higher preferences for the rewarded odor reflect associative learning - presented as a performance index (PI). The conclusion regarding the associative nature of the performance index is compelling, because apart from the contingency between odors and tastants, other parameters, such as odor and reward exposure, passage of time and handling do not differ between the two groups9.

Appetitive Associative Olfactory Learning in Drosophila Larvae

Drosophila larvae are able to associate odor stimuli with gustatory reward. Here we describe a simple behavioral paradigm that allows the analysis of appetitive associative olfactory learning.

에 Appetitive 연관 강한 학습<em&gt; Drosophila</em&gt; 애벌레

In the following we describe the methodological  details of appetitive associative olfactory learning in Drosophila larvae. The setup, in combination with ...

연구

신경과학

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

먹이 찾아 돌아다 행동에 대한 단일 비행 분석에<em&gt; 초파리</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Testing Drosophila Olfaction with a Y-maze Assay

Detecting signals from the environment is essential for animals to ensure their survival. To this aim, they use environmental cues such as vision, mechanoreception, hearing, and chemoperception through taste, via direct contact or through olfaction, which represents the response to a volatile molecule acting at longer range. Volatile chemical molecules are very important signals for most animals in the detection of danger, a source of food, or to communicate between individuals. Drosophila melanogaster is one of the most common biological models for scientists to explore the cellular and molecular basis of olfaction. In order to highlight olfactory abilities of this small insect, we describe a modified choice protocol based on the Y-maze test classically used with mice. Data obtained with Y-mazes give valuable information to better understand how animals deal with their perpetually changing environment. We introduce a step-by-step protocol to study the impact of odorants on fly exploratory response using this Y-maze assay.

Testing Drosophila Olfaction with a Y-maze Assay

We present a choice test to reveal the influence of odorants on Drosophila behavior using a Y-maze assay.

테스트<em&gt; 초파리</emY-미로 분석과&gt; 후각

Detecting signals from the environment is essential for animals to ensure their survival. To this aim, they use environmental cues such as vision, ...

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. When selecting sites to deposit their eggs, female flies are capable of ranking the relative attractiveness of their options and choosing the &#34;greater of two goods.&#34; However, most egg-laying preference assays are not practical if one wants to take a systematic genetic screening approach to search for the circuit basis underlying this simple decision-making process, as they are population-based and laborious to set up. To increase the throughput of studying of egg-laying preferences of single females, we developed custom chambers that each can simultaneously assay egg-laying preferences of up to thirty individual flies as well as a protocol that ensures each female has a high egg-laying rate (so that their preference is readily discernable and more convincing). Our approach is simple to execute and produces very consistent results. Additionally, these chambers can be equipped with different attachments to allow video recording the egg-laying animals and to deliver light for optogenetics studies. This article provides the blueprints for fabricating these chambers and the procedure for preparing the flies to be assayed in these chambers.

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

This protocol describes a high throughput assay for testing egg-laying preferences of Drosophila melanogaster at single-animal resolution. This assay provides a simple, efficient, and scalable platform to identify genes and circuit components that control a simple decision-making process.

높은 처리량 분석은 개인의 알을 낳는 환경 설정을 검사합니다<em&gt; 노랑 초파리</em

Recently, egg-laying preference of Drosophila has emerged as a genetically tractable model to study the neural basis of simple decision-making processes. ...

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools.

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

We describe a protocol for inducing rewarding and nonrewarding experiences in fruit flies (Drosophila melanogaster) using voluntary ethanol consumption as a measure for changes in reward states.

사용 보상 추구 행동의 변화를 측정하는 간단한 방법<em&gt; 노랑 초파리</em

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We ...

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of the right type and quantity of food therefore has a major influence on quality of life. Research on feeding behavior focuses on the underlying processes that ensure actual feeding and unravels the role of factors regulating internal energy homeostasis and the neuronal bases of decision-making. The model organism Drosophila melanogaster, with its great variety of genetically traceable tools for labeling and manipulating single neurons, allows mapping of neuronal networks and identification of molecular signaling cascades involved in the regulation of food intake. This report demonstrates the CApillary FEeder assay (CAFE) and shows how to measure food intake in a group of flies for time spans ranging from hours to days. This easy-to-use assay consists of glass capillaries filled with liquid food that flies can freely access and feed on. Food consumption in the assay is accurately determined using simple measurement tools. Herein we describe step-by-step the method from setup to successful execution of the CAFE assay, and provide practical examples to analyze the food intake of a group of flies under controlled conditions. The reader is guided through possible limitations of the assay, and advantages and disadvantages of the method compared to other feeding assays in D. melanogaster are evaluated.

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake.

모세관 공급 분석은 식품 섭취를 측정<em&gt; 노랑 초파리</em

For most animals, feeding is an essential behavior for securing survival, and it influences development, locomotion, health and reproduction. Ingestion of ...

A Rapid Food-Preference Assay in Drosophila

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate their food environment. The fruit fly, Drosophila melanogaster, is a genetically tractable model organism that is widely used to decipher the molecular, cellular, and neural underpinnings of food preference. To analyze fly food preference, a robust feeding method is needed. Described here is a two-choice feeding assay, which is rigorous, cost-saving, and fast. The assay is Petri-dish-based and involves the addition of two different foods supplemented with blue or red dye to the two halves of the dish. Then, ~70 prestarved, 2-4-day-old flies are placed in the dish and&#160;allowed to choose between blue and red foods in the dark for about 90 min. Examination of the abdomen of each fly is followed by the calculation of the preference index. In contrast to multiwell plates, each Petri dish takes only ~20 s to fill and saves time and effort. This feeding assay can be employed to quickly determine whether flies like or dislike a particular food.

A Rapid Food-Preference Assay in Drosophila

We present a protocol for a two-choice feeding assay for flies. This feeding assay is fast and easy to run and is suitable not only for small-scale laboratory research, but also for high-throughput behavioral screens in flies.

드로소필라의 신속한 식품 선호 분석

To select food with nutritional value while avoiding the consumption of harmful agents, animals need a sophisticated and robust taste system to evaluate ...

Feeding Test: Assessment of Feeding Preferences in Drosophila melanogaster

Source: Bovier, T. F., et al. <a href="https://www.jove.com/video/59535/" target="_blank">Methods to Test Endocrine Disruption in Drosophila melanogaster</a>. J. Vis. Exp. (2019).
This video describes a feeding assay for fruit flies in which colored food is used to monitor feeding and the effect added compounds may have on feeding activity. The example protocol tests the effects of several endocrine disrupting chemicals (EDC).

수유 테스트: Drosophila melanogaster의 수유 선호도 평가

Source: Bovier, T. F., et al. Methods to Test Endocrine Disruption in Drosophila melanogaster. J. Vis. Exp. (2019).
This video describes a feeding assay ...

JoVE Encyclopedia of Experiments

생물학

Drosophila melanogaster (fruit fly)

Adult

Temperature Gradient Assay: A Method to Test Temperature Preference in Drosophila Larvae

Source: Liu, J., et al. <a href="https://www.jove.com/video/57963/" target="_blank">A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae</a>. J. Vis. Exp. (2018).
Drosophila fruit flies can distinguish small changes in temperature and therefore select environments with favorable thermal conditions. This video describes a behavioral assay that tests the temperature preference of Drosophila larvae on a linear thermal gradient.

온도 구배 분석: 초파리 유충의 온도 선호도를 테스트하는 방법

Source: Liu, J., et al. A Temperature Gradient Assay to Determine Thermal Preferences of Drosophila Larvae. J. Vis. Exp. (2018).
Drosophila fruit flies ...

Larva

Taste Preference Assay: A Method for Measuring Feeding Behavior in Drosophila

Source: Bantel, A. P. and Tessier, C. R. <a href="https://www.jove.com/video/54403/" target="_blank">Taste Preference Assay for Adult Drosophila</a>. J. Vis. Exp. (2016).
This video describes the taste preference assay, a behavioral method used to measure attraction or avoidance towards colored solutions that taste differently by assessing the fly&#39;s abdominal coloration after ingestion of the preferred substance. The featured protocol demonstrates the procedure used to measure flies&#39; preference towards solutions of varying sucrose concentrations.

Taste Preference Assay: 초파리의 섭식 행동을 측정하는 방법

Source: Bantel, A. P. and Tessier, C. R. Taste Preference Assay for Adult Drosophila. J. Vis. Exp. (2016).
This video describes the taste preference ...

성인을위한 맛의 기본 설정 분석

요약

더 많은 비디오 탐색

이 비디오의 챕터

에 Appetitive 연관 강한 학습

먹이 찾아 돌아다 행동에 대한 단일 비행 분석에

테스트

높은 처리량 분석은 개인의 알을 낳는 환경 설정을 검사합니다

사용 보상 추구 행동의 변화를 측정하는 간단한 방법

모세관 공급 분석은 식품 섭취를 측정

드로소필라의 신속한 식품 선호 분석

수유 테스트: Drosophila melanogaster의 수유 선호도 평가

온도 구배 분석: 초파리 유충의 온도 선호도를 테스트하는 방법

Taste Preference Assay: 초파리의 섭식 행동을 측정하는 방법