December 17th, 2016
•This protocol uses both subunit coexpression and postlysis subunit mixing for a more thorough examination of recombinant proteasome assembly.
Tags
Videos relacionados
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados