Establishment of the 3D Cell Culture Insert System
1:28
Processing the Filters for Pellet Banking
3:17
Processing the Filters for RNA or DNA Isolation
4:20
Processing the Filters for Protein Isolation
5:42
Processing for H&E and Immunostaining
7:42
Results: Representative Bright Field and Immunofluorescence Images of the Cells Sectioned on the Filter
8:22
Conclusion
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The overall goal of this filter insert-based 3D culture system for patient derived primary cells is to establish a more biologically relevant in vitro model system for prostate cancer research. By using primary human cells, this method can help an
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Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.