Venous Line Preparation and Chronic Indwelling Catheter Placement
5:06
Cecal Ligation and Puncture (CLP)
7:06
Results: Representative Survival Curves after 5 or 7 Days of Critical Illness
8:08
Conclusion
Transcribir
The overall goal of this centrally categorized mouse method for cecal ligation and puncture, or CLP, is to create a reproducible model of prolonged critical illness with intensive care measures to mimic the human clinical setting. This method can
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This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.