August 14th, 2017
•Aedes aegypti mosquitoes infected with Wolbachia are being released into natural populations to suppress the transmission of arboviruses. We describe methods to rear Ae. aegypti with Wolbachia infections in the laboratory for experiments and field release, taking precautions to minimize laboratory adaptation and selection.
Tags
Videos relacionados
Wolbachia Bacterial Infection in Drosophila
Injection of dsRNA into Female A. aegypti Mosquitos
Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Maintaining Wolbachia in Cell-free Medium
Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes
Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes
Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti
Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados