Zebrafish Egg Production and Incubation for Xenograft Assay
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Compound-treated Cell Preparation and Microinjection of Cancer Cells for Xenograft Formation
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Results: Inhibitory Potential of Anticancer Compounds on Spheroid Formation and In Vivo Tumor Formation in Zebrafish
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Conclusion
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This method can help answer key questions in the field of drug discovery to identify anti-cancer effect and gain better mechanistic insight into novel molecules using in vitro and in vivo 3D culture systems. The main advantage of this technique is
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Here, we present the preclinical screening of anticancer coumarins using 3D culture and zebrafish.