Results: PIGF-overexpressing Heart Tissue and miRNA-182 Expression
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Conclusion
Transcripción
This method can help answer key questions in the microRNA field, by providing tools to detect the relative lcalization of both protein and microRNA molecules. The main advantage of this technique is that you can detect both protein and microRNA mo
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micro-RNAs (miRNAs) are short and highly homologous RNA sequences, serving as post-transcriptional regulators of messenger RNAs (mRNAs). Current miRNA detection methods vary in sensitivity and specificity. We describe a protocol that combines in situ hybridization and immunostaining for concurrent detection of miRNA and protein molecules on mouse heart tissue sections.