April 4th, 2019
•We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.
Videos relacionados
Generation of Recombinant Influenza Virus from Plasmid DNA
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Production and Titering of Recombinant Adeno-associated Viral Vectors
Protein Misfolding Cyclic Amplification of Prions
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados