Tumorsphere Preparation for Allograft Transplantation
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Results: Tumorsphere Derivation and Transfection
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Conclusion
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Rhabdomyosarcoma is a rare form of soft-tissue sarcomas characterized histologically by tissue morphology and the expression of myogenic markers. However, given the existence of different subtypes of these tumors and the heterogeneity of the stimu
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This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.