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JoVE Journal

Immunology and Infection

This content is Open Access.

Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization

9.6K Views

06:33 min

October 29th, 2019

October 29th, 2019

9,551 Views

0:04

Title

0:22

Slide Preparation

1:41

Imaging

3:05

Cell Reconstruction

5:10

Results: Representative Assessment of Protein Localization to Specific Cellular Geometries

6:00

Conclusion

Transcribir

This protocol explains how to prepare and mount bacterial samples for live three-dimensional imaging and how to reconstruct the three-dimensional shape of E. coli from those images.

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