February 16th, 2020
•In this protocol, a method of murine islet isolation and transplantation into the inguinal subcutaneous white adipose tissue is described. Isolated syngeneic murine islets are transplanted into a murine recipient using a basement membrane hydrogel. The blood glucose level of the recipients is monitored, and histology analysis of the islet grafts is performed.
Videos relacionados
Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation
Murine Corneal Transplantation: A Model to Study the Most Common Form of Solid Organ Transplantation
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados