Results: Representative Human Kidney Regional Transcriptomics Analyses
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Conclusion
Transcripción
Laser microdissection allows for the transcriptomic analysis of spatially defined regions within the kidney tissue samples. This enables us the unique opportunity to identify structures of interest even after disease induced changes took place. Th
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We describe a protocol for laser microdissection of sub-segments of the human kidney, including the glomerulus, proximal tubule, thick ascending limb, collecting duct and interstitium. The RNA is then isolated from the obtained compartments and RNA sequencing is carried out to determine changes in the transcriptomic signature within each sub-segment.