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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

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09:09 min

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April 12th, 2020

DOI :

10.3791/60939-v

April 12th, 2020

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6,600 Views

Andrea Comba^1,2,4, Patrick J. Dunn^1,2,4, Phillip E. Kish^1,3, Padma Kadiyala^1,2,4, Alon Kahana³, Maria G. Castro^1,2,4, Pedro R. Lowenstein^1,2,4

¹Dept. of Neurosurgery, University of Michigan Medical School, ²Dept. of Cell and Developmental Biology, University of Michigan Medical School, ³Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, ⁴Rogel Cancer Center, University of Michigan Medical School

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Laser Microdissection

Intratumor Heterogeneity

Mesenchymal Cells

Tumor Morphology

Molecular Profiles

Tumor Progression

Therapeutic Targets

Bioluminescence

Cryopreservation

Frozen Brain Tissue

Spatial Characterization

Molecular Characterization

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