Ex Vivo Prep and Exogenous Addition of Metabolites to the Gut Homogenate Extracts for Hyphal Morphogenesis Assay
4:21
C. albicans Morphogenesis Assay
5:50
Results: Effects of Antibiotic and Gut Metabolites on C. albicans Hyphal Morphogenesis
6:58
Conclusion
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The protocol demonstrated in this video allows us to understand the role of gut metabolites on fungal hyphal morphogenesis. This ex vivo technique more or less the closest approximation of the gut for studying fungal hyphal morphogenesis in a way
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The ex vivo assay described in this study using gut homogenate extracts and immunofluorescence staining represents a novel method to examine the hyphal morphogenesis of Candida albicans in the GI tract. This method can be utilized to investigate the environmental signals regulating morphogenetic transition in the gut.