Fluorescence-based Quantitative Real-time PCR (qPCR) and Post-qPCR Analysis by HRM
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Agarose Gel Electrophoresis
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Results: Detection of the Genetic Variants in the CALR Gene
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Conclusion
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This method accurately defines calreticulin mutations in patients with suspected hematologic condition, especially where other diagnostic options are limited or there is an overlap with non-hematologic conditions. The main advantage of the high re
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High resolution melting analysis (HRM) is a sensitive and rapid solution for genetic variant detection. It depends on sequence differences that result in heteroduplexes changing the shape of the melting curve. By combing HRM and agarose gel electrophoresis, different types of genetic variants such as indels can be identified.