In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages

DOI :

10.3791/62497-v

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September 25th, 2021

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3,052 Views

Suryo Kuncorojakti^1,2*, Watchareewan Rodprasert^1*, Quynh Dang Le^1,5, Thanaphum Osathanon^3,4, Prasit Pavasant^3,4, Chenphop Sawangmake^1,5,6

¹Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, ²Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Universitas Airlangga, ³Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, ⁴Center of Excellence in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, ⁵Veterinary Stem Cell and Bioengineering Research Unit, Faculty of Veterinary Science, Chulalongkorn University, ⁶Department of Pharmacology, Faculty of Veterinary Science, Chulalongkorn University

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This protocol presents a comparison between two different induction protocols for differentiating human dental pulp stem cells (hDPSCs) toward pancreatic lineages in vitro: the integrative protocol and the non-integrative protocol. The integrative protocol generates more insulin producing cells (IPCs).

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