Results: Implications of VivoFollow Drift Correction Software
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Conclusion
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Our protocol allows researchers to analyze the process of arteriogenesis in vivo by tracking immune cell adherence and extravasation in growing collateral arteries in real time. The multiphoton microscopy technique allows for the visualization of
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The recruitment of leukocytes and platelets constitutes an essential component necessary for the effective growth of collateral arteries during arteriogenesis. Multiphoton microscopy is an efficient tool for tracking cell dynamics with high spatio-temporal resolution in vivo and less photo-toxicity to study leukocyte recruitment and extravasation during arteriogenesis.