Confocal Laser Scanning Microscopy Imaging of Cleared Tissues
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Results: Neuronal Imaging in Mouse Brain Slice Cleared with Sca/eSF
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Conclusion
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Our protocol would facilitate investigation of neuronal structures from circuit to component scales which is essential for better understanding of brain functions. Our clearing technique, ScaleSF exerts potent clearing capability, as well as a hig
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The protocol provides a detailed method of neuronal imaging in brain slice using a tissue clearing method, ScaleSF. The protocol includes brain tissue preparation, tissue clarification, handling of cleared slices and confocal laser scanning microscopy imaging of neuronal structures from mesoscopic to microscopic levels.