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Axoplasm Isolation from Rat Sciatic Nerve

DOI :

10.3791/2087-v

September 24th, 2010

September 24th, 2010

13,338 Views

1Department of Biological Chemistry, Weizmann Institute of Science

We demonstrate a protocol for axoplasm isolation from adult rat sciatic nerve based on dissection of nerve fascicles and incubation in hypotonic medium to release myelin and lyse non-axonal structures, followed by extraction of the remaining axon-enriched material.

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Axoplasm Isolation

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