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08:32 min
September 10th, 2012
DOI :
10.3791/4230-v
Chapitres
0:05
Title
1:13
Preparing Spleen Tissue
1:57
Preparing Human Islets for Storage
2:43
Preparing Tissue Extract
4:47
Freeze Drying Extracts
6:17
Results: Functional T cell Assays of Extracts
8:08
Conclusion
Résumé
A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
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