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Extraction of Tissue Antigens for Functional Assays

DOI :

10.3791/4230-v

September 10th, 2012

September 10th, 2012

10,658 Views

1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne

A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.

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