S'identifier

JoVE Journal

Biology

Author Produced

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Isolation and Physiological Analysis of Mouse Cardiomyocytes

22.7K Views

11:02 min

September 7th, 2014

September 7th, 2014

22,722 Views

0:00

Title

1:20

Introduction

1:37

Preparation of Reagents and Langendorff Apparatus

3:11

Mouse Preparation and Removal of Heart

3:39

Aorta Cannulation and Langendorf Perfusion

5:25

Myocyte Dissociation and Calcium Tolerance

7:06

Loading Cells with Fura-2

8:00

Microscope Setup

9:01

Measurement and Analysis of Contractility and Calcium Transients

10:39

Conclusion

Individual cardiomyocytes from wild type and mutant mice can be isolated from the heart in order to study their contractility and calcium transients. This allows characterization of the contribution of cellular dysfunction to heart dysfunction from any cause.

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