Results: Neuronal Function in Specific Regions of the Larval Brain Is Necessary and Sufficient for Body Fat Regulation
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Conclusion
Transcription
The overall goal of this assay is to determine any changes in fat storage levels that may be caused by a change in genetics, diet, or treatment. These methods can help answer key questions in the field of metabolism such as genetic or neuronal con
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Here we present a method to measure organismal fat levels in the third instar (L3) larval stage of Drosophila melanogaster. This method exploits the comparatively low density of fat tissue to differentiate between larvae with altered fat stores. Buoyancy-based analysis is a valuable tool for rapid, reproducible, and economical screening.