Imaging Single Vesicle Insertion Events in Live Cells
5:44
Image Analysis and Data Processing
8:17
Results: Monitoring the Localization, Expression, and Trafficking of Membrane Proteins
9:30
Conclusion
Transcription
The overall goal of this fluorescence imaging method is to monitor changes in membrane protein trafficking and intracellular distribution. This method provides insight into changes in protein trafficking, such as the effect of genetic mutations or
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Labeling the extracellular domain of a membrane protein with a pH sensitive fluorophore, superecliptic pHluorin (SEP), allows subcellular localization, expression, and trafficking to be determined. Imaging SEP-labeled proteins with total internal reflection fluorescence microscopy (TIRFM) enables the quantification of protein levels in the peripheral ER and plasma membrane.