Intraventricular Plasmid Injections and Ex Vivo Electroporation of the MGE
3:53
Brain Dissection and Organotypic Slice Cultures
7:16
Results: Time-lapse Live-imaging of Electroporated INs in Organotypic Slices Cultured for 48 h.
9:09
Conclusion
Transcription
The overall goal of this experiment is to visualize genetically modified cortical GABAergic interneurons during tangential migration in the most naturalistic environment possible. This method can help answer key questions in developmental neurosci
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Here, we provide a low-cost and reliable method to generate electroporated brain organotypic slice cultures from mouse embryos suitable for confocal microscopy and live-imaging techniques.