Collecting Intestines and Assessing Bacterial Colonization
4:27
Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
6:43
Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
7:35
Conclusion
Transcription
This method can help answer key questions in the vibrio cholerae field, such as how the vibrio cholerae colonizes in the Quiet Coast and competes with the intestinal microbiota to establish a niche. The main advantage of this technique is that fis
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Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.