Results: Analysis of the Isolated Primary Murine Skeletal Muscle Microvascular Endothelial Cells
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Conclusion
Transcription
This protocol describes a platform technique to isolate microvascular endothelial cells from skeletal muscles. These cells can be used, for example, to gain further insights into blood muscle barrier functions. The main advantage of this technique
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Microvascular endothelial cells of skeletal muscles (MMEC) shape the inner wall of muscle capillaries and regulate both, exchange of fluids/molecules and migration of (immune) cells between muscle tissue and blood. Isolation of primary murine MMEC, as described here, enables comprehensive in vitro investigations of the "myovascular unit".