Generation of LN215-YFPQL and LN215-I" Cells by Lentiviral Transduction
3:14
Plating the LN215-YFPQL and LN215-I‐ Cells
4:42
Conducting the I-YFP GJIC Assay
7:37
Results: Analysis of the Iodine-Yellow Fluorescent Protein-Gap Junction Intercellular Communication Assay
8:15
Conclusion
Transcription
Gap junctions have been suggested as drug targets. Iodide-YFP-GJIC assay is compatible with high-throughput screening to discover gap junction modulators. It have be used to test the effects of a large number of compounds on gap junction activitie
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Here, we present a protocol for a novel gap junction intercellular communication assay designed for the high-throughput screening of gap junction-modulating chemicals for drug discovery and toxicological assessment.