RNA Isolation by Guanidinium Thiocyanate-phenol Extraction and cDNA Library Generation by Reverse Transcription
4:35
Mitochondrial Gene Amplification, Purification, Sequencing and Comparison
7:27
Applying the cDNA Library for Cloning the Genes MgUGD and MgUXS
8:42
Expression and Activity Tests of MgUGD and MgUXS
10:32
Results: Sequencing and Mass Spectrometry Results
11:33
Conclusion
Transcription
The key question we want to address with this method is how can we use RNA samples from material purchased at local food markets for the cloning of biotechnologically relevant genes? Here, we use Pacific oysters as a case study for demonstrating t
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We describe a strategy for how to use RNA samples from unreferenced Pacific oyster specimens, and evaluate the genetic material by comparison with publicly available genome data to generate a virtually sequenced cDNA library.